Tongjiu Hydrazine Hydrate Industr Co.,Ltd

ACTIVATION WITH HYDRAZINE HYDRATE

    Individually, each gel in 35mm dish
    Pour enough hydrazine hydrate over the gel-covets lip to account for evaporation
    Cover with a lid
    Let sit for at least 4 hours
    Move gel-covers lip to new dish with 5% acetic acid (2.5 nil for 50m1 tube). Small dishes should be placed iii a bigger plastic dish.
    Wash on shaker for l ln-
    Move gel-covers lip to new dish with H2Odd
    Wash on shaker for 11u
    Before placid each gel-coverslip into its new dish, raise front mid back with H2Odd
    Store activated gels in PBS@40C

    In bulk, 11gels at a time
    Place each coverslip with gel carefully on custom-made glass rack (takes 11 gels)
    Fill rectangular glass "It- It" dish with -150m1 hydrazine hydrate
    Gently lower glass rack into hydrazine hydrate, let sit for at least 4 hours
    Fill rectangular glass "5% ac.ac." dish with 5% acetic acid (7.5 ml for 150m1)
    Take glass rack out ofh-h and gently lower it into dish with ac.ac.
    Place entire dish into large 150mm plastic dish and put it on shaker
    Wash on shaker for llu"
    Fill rectangular glass "H2O" dish with H2Odd
    Take glass rack out of ac.ac. and gently lower it into dish with water
    Wash on shaker for llu"
    Rinse front aid back of each gel-coverslip with H2Odd froru squat-bottle aid place into 35mm dish
    Store activated gels in PBS@40C
    NOTE: Put both li-li and ac.ac. remains into H-H waste bottle aid record amounts. Raise all dishes
    after use fast with ac.ac.aid then with H2O dd. Always wear gloves!